The synergistic compounds contained in IGF-2 stimulate a variety of complex reactions and messenger systems in the body, and have been clinically proven to increase testosterone production. IGF-2 contains large amounts of Spirosta-steroidal saponins which are intermediates to numerous hormonal pathways in the body, including testosterone production. When ingested, desoxydiosgenin that is contained in IGF-2 is enzymatically converted to 11-oxa-5 alpha-androstane-3,17-dione, a portion of which is then converted to testosterone through the testes. Increased levels of testosterone signals the muscles to increase protein synthesis and nitrogen retention, leading to greater strength, size and recovery.

IGF-2 drives other dynamic biomechanisms including L-Dopa-induced growth hormone release, and increased testosterone secretion through prolactin inhibition. Prolactin is a hormone that regulates testosterone production, and too much of it can suppress the secretion of gonadotropins, which ultimately hinders testosterone production. The variety of Mucuna Pruriens used in IGF-2 is standardized to 25% L-Dopa, delivering large amounts of this active ingredient per capsule. L-Dopa is a precursor to Dopamine, a powerful biogenic amine, and L-Dopa is the only method of transport of Dopamine through the blood-barrier. L-Dopa-induced GHRH release (growth hormone releasing hormone) signals the pituitary to release HGH. HGH is essentially a polypeptide hormone that stimulates skeletal muscle cells to grow and divide; providing for significant, permanent muscle hypertrophy in adults. HGH also aids in cellular recovery and acts synergistically with testosterone to provide a highly anabolic muscle-building environment.

IGF-2 also stimulates the production of cyclic AMP (cAMP) from ATP. cAMP generation has a variety of effects on tissues, but the most notable is the stimulation of the thyroid to produce thyroxin, which promotes fat metabolization. The increased cAMP levels activate the protein kinase enzyme, which in turn activates hormone-sensitive lipase (HSL) in target tissues. cAMP also signals for the production of luteinizing hormone (LH), which has been shown to elevate testosterone levels in males. Elevated levels of cAMP and LH stimulate a marked increase in the use of fat as body fuel, and are strongly correlated to increased testosterone production.